Opto B Discovery Application

Fuel Antibody Discovery
Breakthroughs with
Function-First Screening

OVERVIEW
Powering Your Success in Single B Cell Antibody Discovery with the Opto® B Discovery Application

Opto B Discovery on the Beacon® platform revolutionizes antibody discovery (AbD) with its function-first, high-throughput single B cell screening, maximizing the success of your antibody campaigns.​​

By conducting multiple functional assays, including antigen specificity, affinity, cross-reactivity, cell-based functions, and blocking assays, on tens of thousands of cells in a single run, the Beacon platform delivers the highest level of characterization for each single cell.​​

The Opto B Discovery workflows on the Beacon platform provides an unparalleled, efficient, and reliable solution for antibody discovery, ensuring your success with both easy and challenging targets.

Opto B Discovery Application

Revolutionizing Antibody Discovery

Supported Instruments: Beacon, Beacon Select for AbD, Beacon Quest
Capabilities
Develop a Deeper Understanding
of Antibody Discovery with the Beacon Platform​

Prioritize functionality first

Vast Portfolio of Flexible, Function-First Assays to Generate Rich, High-Parameter Data​

Conduct up to 16 functional assays, including antigen specificity, affinity, cross-reactivity, ligand blocking, internalization, and cell-based function, on up to 60,000 B cells per run, generating up to 1 million data points and maximizing antibody characterization. Empower artificial intelligence (AI)-based antibody discovery by leveraging the rich, high-parameter data generated by the Beacon platform to train data-hungry AI models.

Antigen Specificity Assay
Antigen Specificity Assay
 
Cross-reactivity and Affinity Assays
Cross-reactivity and Affinity Assays
 
Membrane-bound Antigen Cell-binding Assay
Membrane-bound Antigen Cell-binding Assay
 
Ligand Blocking Assay
Ligand Blocking Assay
Affinity Assay
AbD Page Affinity_Schematic
 
Affinity Assay
Boost Your Hit Generation with Early Antibody Affinity Ranking

Optimize your antibody discovery process with the innovative Opto® B Discovery Affinity Assay. This cutting-edge tool allows researchers to rapidly rank antibody hits based on relative affinity, enhancing the selection of top candidates early in the discovery process.

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Webinar

Multiplexed and Quantitative Assays for Enhancing Antibody DiscoveryDr. Kate Malecek, Director of Beacon Center for Single Cell Biology at Caltech

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App Note

Rapid High-Resolution Screening of Plasma B Cells to Identify Antibody Lead Candidates

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Maximize discovery success

Antibody Screening for Diverse and Challenging Targets​

Screen antibodies across diverse species (human, mouse, rabbit, alpaca, and more), organ types (spleen, bone marrow, lymph nodes, and peripheral blood) and cell types (plasma and memory B cells), resulting in diverse antibody hits with even the most challenging targets, including multi-transmembrane proteins, targets with high host homology, and low immunogenicity antigens.

Screen Antibodies Across
Diverse Host Species
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Generate Antibody Hits
for Challenging Targets
Multi-transmembrane Proteins​
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Learn More
Webinar

Applying the Beacon Platform to a GPCR CampaignFrançois Romagné, Scientific Director, MImAbs

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App Note

Opto Memory B Discovery Enables Multispecies Screening Capabilities

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Expand assay applicability

Versatile Assays Applicable Across a Wide Range of Research Areas

Empower screening with small-volume samples and customizable assays suitable for therapeutic antibodies in any disease area. Optofluidics are ideal for small or precious human samples (as few as a single patient draw) and enable broad applications, ranging from vaccine development for infectious diseases, discovering autoantibodies responsible for autoimmune disorders, and identifying novel antibodies against tumor-associated antigens (TAAs) for oncology.

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App Note

Opto Memory B Discovery Human Workflow

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Publication / Science

Transcobalamin receptor antibodies in autoimmune vitamin B12 central deficiency​

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Publication / Cell

Natural malaria infection elicits rare but potent neutralizing antibodies to the blood-stage antigen RH5​

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Fast and automated workflow

Offering the Fastest Path From Sample to Lead​

Streamline antibody discovery by minimizing screening time and expenses. B cells can be screened for functionality in just one day, allowing the advancement of only the most promising candidates. The single B cell BCR recovery & library prep kits convert exported hits into antibody sequences within a week. Additionally, one-step cDNA cloning facilitates the rapid expression of recovered antibodies, promoting efficient lead characterization and selection. ​

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Publication / Nature Medicine

Rapid isolation and profiling of a diverse panel of human monoclonal antibodies targeting the SARS-CoV-2 spike protein

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Video

Hear the story behind the publication from Dr. James Crowe, Vanderbilt University: How Art Inspired the Development of the Covid-19 Vaccine

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Meet the Beacon Optofluidic System
The Most Advanced Antibody Discovery Platform
Function-First Total Solution for Rapid Antibody Discovery

At the core of the Beacon system is a combination of optics and nanofluidics called optofluidics. The OptoSelect® chips replace typical well plates. Each OptoSelect chip contains thousands of NanoPen® chambers, which replace wells on a microplate. This is where cells are selectively placed, cultured and characterized using a myriad of proprietary Beacon Platform assays.

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Key Product Features
  • Comprehensive assays​
    1 million or more multi-modal data points for specificity, cross-reactivity, affinity, blocking, and function​
  • Broad species compatibility​
    Total end-to-end solutions for mouse, rabbit, human, and alpaca, with a rapid path to onboard others (e.g. rat, chicken)​
  • High-throughput screening​
    Up to 60,000 single cells screened for every assay you choose​
  • Automated sequence recovery ​
    Recover 192 to 1000s of paired heavy and light chains​
  • Rapid single-day workflow
How It Works

Understand the life of single cells through functional cellular analysis with the Beacon Platform.

Customized Cell Selection: Exquisite control of single cells maximizes antibody discovery success.
Breadth of Functional Assays: Function-first assays enable deep characterization of single B cells in multiple sequential assays.
Highest Parameter Functional Data: Integrated data analysis lets you link cellular function with genomics and transcriptomics to understand underlying molecular mechanisms.
Antibody Discovery with a Function-First Approach

See how innovators in antibody discovery leveage the vast portfolio of flexible, function-first assays
on the Beacon platform to generate rich, high-parameter data and maximize antibody characterization

Researcher Spotlights
Bruker Cellular Analysis' Functional Cell Biology Has Been Published in 250+ Publications
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Resources
Research & Datasheets
App Note
Abveris on Antibody Discovery
App Note
Rapid High-Resolution Screening of Plasma B Cells to Identify Antibody Lead Candidates
Customer Spotlight
Amgen on Antibody Discovery and Cell Line Development
Beacon for Antibody Discovery
Let’s Unleash the Full Potential of
Your Antibody Discovery Research

Complete the form to connect with us and discover how the Beacon technology can enhance your Antibody Discovery programs.

Our expert team will be able to answer your questions, show you how the Beacon system works, and share the most relevant datasheets & case studies for your research.