Optofluidic Chips

T Cell Profiling Kit

Thoroughly profile single T cells and link their phenotypic and
functional data to their genotypes.

OVERVIEW
T Cell Profiling Kit, a Comprehensive Solution That Empowers You to Thoroughly Profile Single T Cells and Link Their Phenotypic and Functional Data to Their Genotypes

With this kit, you can simultaneously investigate the functional aspects of T cells as they interact with target cells, gaining valuable insights into the diverse mechanisms that regulate cytokine secretion using customizable cytokine assays and cell surface markers. The kit enables the characterization of polyfunctionality, cytotoxicity, genotype, and gene expression, providing a complete understanding of T cell behavior. Furthermore, you can sort, select, and load cells, making it possible to isolate desired phenotypes from heterogeneous T cell samples.

Our cytotoxicity assay, helps visualize distinct killing behaviors in your T cell samples. Once the functional characterization is complete, cells of interest can be exported for expansion or further study, facilitating the linkage of their behavior to genotype.

Product Guide

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Build a Kit
Customize your kit to examine various cell types and assay methods for Immune profiling
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Application & Workflow
Explore T cell behavior, from cytokine secretion to genotype, enabling cell isolation, visualization of killing behaviors, and connecting cell behavior to genotype for further study
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Research & Data
Explore our application notes and publications to see how multi-omic analysis can be used to accelerate your research
Supported Instruments: Beacon®, Beacon Quest™
Measure Multiple Cytokines

Customizable cytokine assays reveal T cell interactions and diverse cytokine secretion mechanisms.

Visualize Tumor Cell Killing

Visualize T cell killing behaviors using caspase-3 activation and identify the cells that matter.

Link Function to Genotype

Recover and sequence your cells to link phenotype and gene expression in a single workflow.

Product Support
View our guides, manuals, and technical support resources.
Explore T Cell Profiling
Applications
TECHNOLOGY
T Cell Profiling Kit

Thoroughly profile single T cells and link their phenotypic and functional data to their genotypes

Compatible Instruments:

T CELL PROFILING PRODUCTS
Build Your Kit

This T cell profiling kit allows you to explore T cell behavior by investigating cytokine secretion, surface markers, polyfunctionality, cytotoxicity, genotype, and gene expression, facilitating cell isolation, visualization of killing behaviors, and subsequent export for expansion or further study, connecting cell behavior to genotype.

Workflow  
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TCR-T

Can be used to perform function-forward screening to identify TCR sequences

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CAR-T

Can be used to screen CAR libraries (DKFZ webinar), validate targets, optimize CAR manufacturing

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Antigen Screens and Activation

As described in the MCW webinar, the Antigen Presenting Bead kit can be used to screen targets, and to then activate and expand T Cells

Assay Types  
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Opto® Antigen Presenting Bead Kit, HLA*02:01 (750-01002)
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Multiplex Cytokine Analysis (MCA)
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Surface Marker Staining
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Cytotoxicity
Chip Type  
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OptoSelect® 3500 Chip (750-00012)​
  • ~3,550 NanoPens per chip​
  • Large pen geometry allows for loading up to 3 multiplexed beads in addition to up to 2 cell types
Sequencing Kits  
TCRseq
TCRseq Well Plate Kit, Human (750-01001)

Used to amplify TCR alpha and beta chains, and prepare them for next-generation sequencing.

RNASeq
OptoSeq® Single Cell 3' mRNA Kit (750-03000)

Used to amplify and sequence cDNA and analyze differential gene expression profiles.

Comprehensive Profiling for functional Insights

Rapid, Single-Cell Multiomic T Cell Profiling

Sort, Select and Load Cells

Select specific phenotypes from varied T cell samples based on markers. Gentle sorting minimizes stress without media exchanges.

Comprehensive Profiling

Characterize polyfunctionality, cytotoxicity, genotype, and gene expression.

Measure Multiple Cytokines

Customizable cytokine assays reveal T cell interactions and diverse cytokine secretion mechanisms.

Visualize Tumor Cell Killing

Visualize T cell killing behaviors using caspase-3 activation and identify the cells that matter.

Link Function to Genotype

Recover and sequence your cells to link phenotype and gene expression in a single workflow.

Automated Data Analysis

Quickly analyze your data with the CAS software platform.

Build Your
T Cell Profiling Kit
Resources
Research & Datasheets
Optofluidic Webinar
Harnessing the Power of Single Cell Technologies to Build More Effective Cell Therapies
Harnessing the Power of Single Cell Technologies to Build More Effective Cell Therapies
Speaker: Anthony E. Zamora, PhD, Assistant Professor, Medicine (Hematology and Oncology) & Microbiology & Immunology
Optofluidic Webinar
Maximize Functional Characterization of Your Precious T Cell Samples
Maximize Functional Characterization of Your Precious T Cell Samples
Speaker: Mark White, PhD, Sr. Director, T Cell Discovery, Berkeley Lights
Optofluidic Webinar
Multi-omic Profiling of T Cells using the OptoSeq® Single Cell 3′ mRNA Kit
Multi-omic Profiling of T Cells using the OptoSeq® Single Cell 3′ mRNA Kit
Speaker: Jason McEwen, PhD, Director of Biology, Berkeley Lights
Brochure
Comprehensive Single T Cell Profiling
App Note
Profile and Recover Polyfunctional T Cells Using Multiplexed Cytokine Secretion Assays
Datasheet
The Opto® Antigen Presenting Bead Kit
Beacon® for T Cell Profiling
Let’s Unleash the Full Potential of
Your T Cell Profiling Research

Complete the form to connect with us and discover how the Beacon® technology can enhance your T Cell Profiling programs.

Our expert team will be able to answer your questions, show you how the Beacon® system works, and share the most relevant datasheets & case studies for your research.