OVERVIEW
Interrogate T Cell Function with the Optoยฎ T Cell Profiling Applicationโ
As the need and demand for more effective therapies expands, the Beacon platform is a key instrument that can be used to accelerate T cell therapeutic development. The Optoยฎ T Cell Profiling workflow on the Beacon platform automates and accelerates this journey by swiftly conducting functional assays across thousands of T cells. Explore single-cell polyfunctionality, cytotoxicity, phenotype, and gene expression with precision sorting and comprehensive profiling. Recover cells of interest for further study, connecting their behavior to genotype and gene expression. Our assays provide you with the greatest depth of functional single-cell profiling to unveil diverse mechanisms in cytokine secretion, immune function, and tumor destruction.
T Cell Profiling Application
Maximize Functional Characterization
Develop a Deeper Understanding
of T Cell Profiling
Accelerate TCR and CAR Discovery with Single T Cell Functional Screeningโ
The Beacon platform allows you to directly screen TCR, CAR, T cell, target cell, patient cell, or peptide libraries to visualize cellular functionality. Screening these libraries on the Beacon platform allows users to precisely co-culture a cell with a target to assess interactions and functionality by measuring cytotoxicity, cytokine secretions, and surface markers. Identifying successful targeting modalities with the Beacon platform takes only 5 days whereas other techniques require multiple weeks or months of effort.โ
Comprehensively Profile the Performance of Single T Cellsโ
When developing adoptive cell therapies, a fundamental step involves validating their functionality against cancer, yet this can be a major bottleneck since it typically requires multiple instruments and multiple experiments to perform different assays on each product the cell generates. The Beacon platform combines multiple functional assays, including cytokine secretion, cytotoxicity, and surface marker staining, to provide direct readouts of cellular functionality at the single cell level. The Beacon platform can reduce the time needed to validate product performance while requiring fewer cells than traditional validation approaches.โ
Cytotoxicity Assay for Cell Therapy Development
Validating the Functional Performance of Immune Cells at a Single-Cell Level
DNAJB1-PRKACA fusion neoantigens elicit rare endogenous T cell responses that potentiate cell therapy for fibrolamellar carcinoma
Uncover Powerful Translational Biomarkers Through Single T Cell Functional Analysisโ
At the clinical translation stage of cell therapy development, donor heterogeneity is a factor that affects efficacy. Profiling single-cell functionality using the Beacon platform can reveal impactful biomarkers that correlate with clinical outcomes by performing functional analysis on cytotoxicity and cytokine secretion with surface marker analysis at the single-cell level. Fully characterizing cellular products provides greater depth and unveils potential biomarkers that can correlate with different clinical outcomes such as response, no response, relapse, remission, and side effects such as cytokine release syndrome and neurotoxicity โ
Leveraging Real-Time Biology to Streamline the Discovery, Validation, and Translation of Cell-Based Therapies for Cancer
Sequence Recovered T Cells to Link Function to Genomicsโ
Sequence recovered cells using TCR-seq or RNA-seq to generate multi-omic insights that link functionality to genomics. After functional characterization, cells of interest can be exported for expansion or for further study to link their behavior to genotype. The TCRseq Well Plate kit allows you to recover your selected cells from fewer than 10,000 cells, amplify TCR alpha and beta chains, and sequence only the T cells that are potential therapeutics. The OptoSeqโข 3โฒ mRNA kit is the first of its kind โ a combined multi-omics workflow which makes it possible to perform cytokine secretion analysis and gene expression profiling on the same cell.โ
Meet the Beacon Optofluidic System
The Most Advanced
T Cell Profiling Platform
Function-First Total Solution for In-Depth T Cell Profiling
At the core of the Beacon system is a combination of optics and nanofluidics called optofluidics. The OptoSelectยฎ chips replace typical well plates. Each OptoSelect chip contains thousands of NanoPenยฎ chambers, which replace wells on a microplate. This is where cells are selectively placed, cultured and characterized using a myriad of proprietary Beacon Platform assays.
Key Product Features
- Exquisite Control: Precisely manipulate cell and bead movements for custom assays
- Live Cell Imaging: Leverage live cell imaging and real-time data for dynamic analysis
- Comprehensive Sequential Assays: Conduct multiple assays on the same live cells on-chip
- Integrated Multiomics: Connect on-chip functional analysis with off-chip transcriptomic & genomic profiling from the same single cells
- Scalable Sample Handling: Adapt to high-throughput screening and analyze precious samples with scalable input
Perform Multiple Assays at Once for Unprecedented
Single-Cell Functional Analysis Insights
Surface Marker Phenotype
Introduce any surface marker stain to your assay
Use surface marker staining to link behaviors such as activation, exhaustion, and persistence with cytotoxicity and cytokine secretionโ
Multiplexed Cytokine Secretion
Assess cytokine secretion with capture beadsโ
Analyze functional cytokine secretion to characterize cellular heterogeneity and polyfunctionalityโ
Dynamic Cytotoxicity
Co-culture a T cell with a target cell to observe cytotoxicity
Use fluorescence to dynamically show when target cell apoptosis occurs across thousands of NanoPensโ
How It Works
Understand the life of single cells through functional cellular analysis with the Beacon Platform.
- Customized Cell Selection: Exquisite control of single cells maximizes T cell profiling success.
- Breadth of Functional Assays: Function-first assays enable deep characterization of single T cells in multiple sequential assays.
- Highest Parameter Functional Data: Integrated data analysis lets you link cellular function with genomics and transcriptomics to understand underlying molecular mechanisms
Advancing Single-Cell Methods in Solid Tumor Oncology with Brukerโs Optofluidic Platform
In this webinar we discuss:
- Establishing a single-cell co-culture system to identify and visualize interactions between cancer cells and T cells in real-time
- Measuring tumor-specific TIL functionality and tumor-killing capacity from biopsies obtained from HNSCC patients
- A powerful strategy to generate new immunotherapies by identifying the TCR sequence of functional TIL cells
Researcher Spotlights
Research & Datasheets
Beacon for T Cell Profiling
Unleash the Full Potential of
Your T Cell Profiling Research
Complete the form to connect with us and discover how the Beacon technology can enhance your T cell profiling research.
Our expert team will be able to answer your questions, show you how the Beacon system works, and share the most relevant datasheets & case studies for your research.