T Cell Profiling
Comprehensive profiling with
recovery of cells of interest
Workflow In Action
See how our customers are applying the T cell workflow to develop a deeper understanding of T cell biology.
Harnessing the Power of Single Cell Technologies to Build More Effective CAR-T Cell Therapies
Maximize Functional Characterization of Your Precious T Cell Samples
The Opto® T Cell Profiling workflow accelerates the creation of better therapeutics by performing multiple functional assays across thousands of individual T cells in a matter of days. Deeply characterize, profile, and map your T cell populations at the single-cell level to directly link phenotype to gene expression.
One T cell could be the key to the next powerful therapeutic.
To realize its potential, you need to deeply characterize it. The Opto T Cell Profiling workflow lets you directly link single-cell phenotype and function to gene expression. It only takes one cell to create a powerful therapy. We’ll help you find it.
— Cruickshank Quinn
Allowing desired phenotypes to be selected from heterogeneous T cells samples
Specific cells are targeted based on size, viability and cell surface markers. This gentle sorting process does not require repeated media exchange, which can reduce sorter induced cell stress.
Characterize polyfunctionality, cytotoxicity, genotype, and gene expression
Our Multiplex Cytokine Assay allows simultaneous functional interrogation of T cells as they interact with target cells, giving you insight into the heterogenous mechanisms that regulate cytokine secretion.
Cytokine assays and cell surface markers can be customized
Our Cytotoxicity Assay leverages caspase-3 activation in target cells to visualize the disparate killing behaviors in your T cell samples.
Now, you can develop therapeutics to mediate more effective tumor destruction by the few T cells that matter.
After functional characterization, cells of interest can be exported for expansion or for further study to link their behavior to genotype.
Link Function to Genotype
The TCRseq Well Plate kit allows you to recover your selected cells from fewer than 10,000 cells, amplify TCR alpha and beta chains, and sequence only the T cells that are potential therapeutics.
Link Phenotype to Gene Expression
The OptoSeq™ 3′ mRNA kit is the first of its kind — a combined multi-omics workflow which makes it possible to perform cytokine secretion analysis and gene expression profiling on the same cell.
The Opto® Antigen Presenting Bead (Opto APB) Kit, HLA*02:01 can be used to measure binding of putative antigenic peptides to HLA Class I, measure stability of peptide-HLA complexes over time, and, in conjunction with costimulatory antibodies pre-conjugated to the APBs, efficiently stimulate and expand endogenous antigen-specific T cells. Antigen-specific T cells are detected using a fluorescently labeled tetramer, which can be purchased directly from MBL International.