T Cell Profiling Application

Comprehensive
Profiling
with Recovery
of Cells of Interest

OVERVIEW
T Cell Profiling

Discover the power within a single T cell - the key to groundbreaking personalized therapeutics. Our Opto® T Cell Profiling workflow accelerates this journey by swiftly conducting functional assays across thousands of T cells. With precision sorting and comprehensive profiling, explore single-cell polyfunctionality, cytotoxicity, genotype, and gene expression. Our multiplexed cytokine and cytotoxicity assays unveil diverse mechanisms in cytokine secretion and tumor destruction. Recover cells of interest for further study, connecting their behavior to genotype and gene expression.

T Cell Profiling Application

Maximize Functional Characterization

Comprehensively profile your T cells at a single-cell level
Visualize functionality using cytotoxicity, cytokine secretion, and surface marker assays
Recover cells of interest to link phenotype to genotype
Supported Instruments: Beacon, Beacon Quest
Technology
Maximize Functional Characterization of Your Precious T Cell Samples

The Opto® T Cell Profiling workflow accelerates the creation of better therapeutics by performing multiple functional assays across thousands of individual T cells in a matter of days. Deeply characterize, profile, and map your T cell populations at the single-cell level to directly link phenotype to gene expression.​

THE IMPORTANCE OF A SINGLE T CELL
One T cell could be the key to the next powerful therapeutic.

To realize its potential, you need to deeply characterize it. The Opto T Cell Profiling workflow lets you directly link single-cell phenotype and function to gene expression. It only takes one cell to create a powerful therapy. We’ll help you find it.

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Customer Webinars
Workflow in Action
Featured
Harnessing the Power of Single Cell Technologies to Build More Effective CAR-T Cell Therapies
Accelerate T Cell Profiling

See how pioneers in T Cell Profiling use our technology to speed up workflows.

Capabilities
Develop a Deeper Understanding
of T Cell Profiling

Accelerate timelines and reduce costs

SORT, SELECT AND LOAD CELLS
Allowing desired phenotypes to be selected from heterogeneous T cells samples

Specific cells are targeted based on size, viability and cell surface markers. This gentle sorting process does not require repeated media exchange, which can reduce sorter induced cell stress.

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COMPREHENSIVE PROFILING
Characterize polyfunctionality, cytotoxicity, genotype, and gene expression

The Opto T Cell Profiling workflow makes it possible to comprehensively profile single T cells and link this phenotypic and functional data to the genotype of those same cells.

Download Datasheets

Multiplex Cytokine Assay Datasheet (PDF)
Cytotoxicity Assay Datasheet (PDF)
TCRseq Kit Datasheet (PDF)

MEASURE MULTIPLE CYTOKINES
Our Multiplex Cytokine Assay allows simultaneous functional interrogation of T cells as they interact with target cells, giving you insight into the heterogenous mechanisms that regulate cytokine secretion.

Cytokine assays and cell surface markers can be customized.

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VISUALIZE TUMOR CELL KILLING
Our Cytotoxicity Assay leverages caspase-3 activation in target cells to visualize the disparate killing behaviors in your T cell samples

Now, you can develop therapeutics to mediate more effective tumor destruction by the few T cells that matter.

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RECOVER CELLS OF INTEREST
After functional characterization, cells of interest can be exported for expansion or for further study to link their behavior to genotype.​
Link Function to Genotype

The TCRseq Well Plate kit allows you to recover your selected cells from fewer than 10,000 cells, amplify TCR alpha and beta chains, and sequence only the T cells that are potential therapeutics.

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Link Phenotype to Gene Expression

The OptoSeq™ 3′ mRNA kit is the first of its kind — a combined multi-omics workflow which makes it possible to perform cytokine secretion analysis and gene expression profiling on the same cell.

Resources
Research & Datasheets
Brochure
Comprehensive Single T Cell Profiling
App Note
Profile and Recover Polyfunctional T Cells Using Multiplexed Cytokine Secretion Assays
Datasheet
The Opto® Antigen Presenting Bead Kit
App Note
Perform Multi-omic Analysis to Directly Link T Cell Polyfunctionality to Gene Expression
Beacon for T Cell Profiling
Let’s Unleash the Full Potential of
Your T Cell Profiling Research

Complete the form to connect with us and discover how the Beacon technology can enhance your T Cell Profiling programs.

Our expert team will be able to answer your questions, show you how the Beacon system works, and share the most relevant datasheets & case studies for your research.