Protocol Training Modules
13 Chapters
IsoCode Single Cell Polyfunctional Strength: CAR-T Protocol
Follow along with the validated CAR-T Sample Preparation Protocol.
Explore by Chapter
Explore by Chapter
CHAPTER 1
2:01
Getting Started
Performing sample preparation for the IsoCode Single Cell Polyfunctional Strength CAR-T Protocol.
CHAPTER 2
2:54
Recovery of Target Cell Lines
Recovering your cryopreserved target cell lines. Perform at least three days prior to the recovery of your CAR-T cells (Chapter 4).
CHAPTER 3
3:07
Culture of Target Cells
Culturing your target cells.
CHAPTER 4
2:59
Recovery of CAR-T Cells
Recovering your cryopreserved CAR-T cells.
CHAPTER 5
1:44
Pre-Sample Enrichment
Preparing your CAR-T cells for sample enrichment.
CHAPTER 6
7:43
CD8 Sample Enrichment
Enriching the CD8 CAR-T cells.
CHAPTER 7
3:58
Cell Stimulation
Stimulating your CAR-T cells with your target cells.
CHAPTER 8
7:17
Target Cell Depletion
Performing target cell depletion for your CAR-T cells.
CHAPTER 9
4:04
Cell Staining
Staining your stimulated and target cell depleted CAR-T cells.
CHAPTER 10
0:46
Chip Thaw
Thawing your IsoCode chips 30-60 minutes prior to chip loading.
CHAPTER 11
1:57
Chip Loading
Loading your CAR-T cells onto the thawed IsoCode chips.
CHAPTER 13 Appendix
3:37
Field Guide - How to Lift Stimulated CD8/CD4 Cells
Watch the Field Guide: How to Lift Stimulated CD8/CD4 Cells
CHAPTER 12 Appendix
3:53
Cell Quantification & Viability
Calculating cell quantification and viability.