Optofluidic chips and reagents

T Cell Profiling Kit

Characterize T Cell Function and
Link Phenotype to Genotype

Download Product Sheet
OVERVIEW

Unlock Deeper Insights into T Cell Function with the T Cell Profiling Kit for the Beacon® Platform

This kit enables you to:

  • Analyze multiple functions simultaneously: Assess dynamic cytotoxicity, surface markers, and multiplexed cytokine secretion in live single cells.
  • Sort and select with precision: Isolate T cells with desired phenotypes from mixed populations.
  • Connect function to genotype: Export selected cells for expansion or further multiomic analysis, including TCR-seq and single-cell mRNA sequencing (scRNA-seq).
  • Gain a comprehensive view of T cell performance: Assess polyfunctionality, cytotoxicity, phenotype, and gene expression.

Product Guide

icon-product-build-sm
Chips & Reagents
Select reagents and chips for your specific T cell profiling assay
icon-product-workflow-sm
Application & Workflow
Explore T cell behavior, from cytokine secretion to cytotoxicity to genotype
icon-resource-graph-46
Research & Data
Explore our application notes and publications to see how optofluidics can be used to functionally profile T cells
icon-product-support-sm
Technical & Product Support
View our guides, manuals, and technical support resources
icon-beacon-outlined-30
Supported Instruments
Beacon®, Beacon Quest™
TECHNOLOGY

T Cell Profiling Kit

Thoroughly profile single T cells and link their phenotypic and functional data to their genotypes

Compatible Instruments:

optofluidic-technology-t-cell-profiling-inner-panel-v3
Applicable Assays
  • Dynamic Cytotoxicity
  • Multiplexed Cytokine Secretion
  • Surface Marker Phenotype
Products

Build Your Kit

To perform a T Cell Profiling assay, you will need to select chips and beads, and, if performing downstream sequencing analysis, a sequencing kit. Speak with a BCA representative to help build your kit.

Available Items
for Your Kit

Chip Type

Chip type needed for T cell profiling assay.

icon-product-chip-white
OptoSelect® 3500 Chip (750-00012)
  • ~3,550 NanoPen chambers per chip​

Bead Type

Conjugate beads with an antigen or peptide for enrichment or co-culture.

opto-antigen-bead-kit-75
Opto® Antigen Presenting Bead Kit, HLA*02:01 (750-01002)

Sequencing Kits

Used to amplify, sequence, and analyze differential gene expression profiles.

TCRseq
TCRseq Well Plate Kit, Human (750-01001)

Used to amplify TCR alpha and beta chains, and prepare them for next-generation sequencing.

RNAseq
OptoSeq® Single Cell 3' mRNA Kit (750-03000)

Used to amplify and sequence cDNA and analyze differential gene expression profiles.

Additional Reagents for Your Assays*

*Not provided by Bruker Cellular Analysis​

Explore T Cell Profiling assays

Viability
  • Viability stains
Cytotoxicity
  • Caspase-3 substrate
Cytokine Secretion
  • Cytokine capture beads
  • Secondary stains
Surface Phenotype
  • Surface marker stains

Click here to see a list of additional reagents and consumables from external suppliers that have been validated by Bruker Cellular Analysis to perform in association with these assays on the Beacon platform for immune cell profiling workflows.

How to Use
Your Kit

Supported Cell Types

opto-bead-50
  • T cell
  • CAR-T cell
  • TCR-T cell
  • TIL cell

Additional Types

Interested in other cell types? Contact us.

Order Your T Cell
Profiling Kit Today
Request a Quote

Comprehensive Profiling for
Functional Insights

Rapid, Single-Cell Multiomic T Cell Profiling

Sort, Select and Load Cells

Select specific phenotypes from varied T cell samples based on markers. Gentle sorting minimizes stress without media exchanges.

Comprehensive Profiling

Characterize polyfunctionality, cytotoxicity, genotype, and gene expression.

Measure Multiple Cytokines

Customizable cytokine assays reveal T cell interactions and diverse cytokine secretion mechanisms.

Visualize Tumor Cell Killing

Visualize T cell killing behaviors using caspase-3 activation and identify the cells that matter.

Link Function to Genotype

Recover and sequence your cells to link phenotype and gene expression in a single workflow.

Automated Data Analysis

Quickly analyze your data with the CAS software platform.

Explore T Cell Profiling
Applications

View Application
Resources

Research & Datasheets

Brochure
Comprehensive Single T Cell Profiling
View Resource
App Note
Identify and Select Optimal CAR T Cell Phenotypes with the Opto® Cell Therapy Development Workflow
View Resource
App Note
Unveiling Critical Translational Biomarkers through Single-Cell Analysis
View Resource
Show More Resources loader
Beacon for T Cell Profiling

Unleash the Full Potential of
Your T Cell Profiling Research

Complete the form to connect with us and discover how the Beacon technology can enhance your T Cell Profiling programs.

Our expert team will be able to answer your questions, show you how the Beacon system works, and share the most relevant datasheets & case studies for your research.