OVERVIEW
Streamline Monoclonal Antibody Discovery with Advanced Reagents and Chips for the Beacon Platform
The Antibody Discovery kit for the Beacon® platform lets you screen plasma cells or memory B cells and find valuable monoclonal antibodies within a week. Feeder-free activation and culture media ensure strong activation of memory B cells and longer secreting plasma cells for reliable results. Choose from a variety OptoSelect® chips to give you the screening throughput you need for simple or difficult targets. Simplify the recovery of antigen-specific antibody sequences for downstream analysis and development using our OptoSeq® BCR Bead Unload and Opto® B Discovery Sanger Prep kits. With the Antibody Discovery products for the Beacon platform, you'll expedite your research and achieve more specific and effective antibody discoveries in the process.
Product Guide
TECHNOLOGY
Antibody Discovery Kit
A powerful automated solution for faster, more precise Antibody Discovery
Compatible Instruments:
- Simple to multiplex and/or perform sequential assays
- Automated assay scoring available
- Requires significantly more optimization than in-channel assays
- Manual assay scoring required
- Useful for low secretion rates and/or long assay readout times (e.g. cytotoxicity, reporter gene)
Products
Build Your Kit
Mix and match antibody discovery products and customize with your preferred reagents and primers to tailor your ideal workflow. Speak with a BCA representative to help build your kit.
Select Your Kit Components
1) Species Sample Prep Kit
Species and cell types supported for screening.
Human
- Memory B cells (750-08323)
- Plasma cells (750-02051)
- Optional Memory B cells PBMC Thawing Kit (750-08254)
Rabbit
- Memory B cells (750-08251)
- Optional Memory B cells PBMC Thawing Kit (750-08254)
Mouse
- Memory B cells (750-08251)
- Plasma cells (750-02050)
- Optional Memory B cells PBMC Thawing Kit (750-08254)
Camelid Just Released
- Memory B cells (KT52-00007)
- Optional Optional Memory B Cells PBMC Thawing Kit (750-08254)
Other Species: Leverage the power and flexibility of single B cell screening to develop your own reagents and .workflows for other species such as cow, chicken, sheep, and goat.
2) Chip Type
Choose a Chip type for your application based on
cell type and assay needs.
OptoSelect® 11k Chip (750-08090)
- ~11,000 NanoPen chambers per chip
OptoSelect® 20k Chip (750-00019)
- ~20,000 NanoPen chambers per chip
3) Assay Preparation
Beads to assess B cell Ig subtype, antigen specificity,
and/or antibody function.
Assay Beads (520-00053)
Anti-Camelid IgG (H+L) Assay Beads (RG52-00026)
4) BCR Recovery Kit
Choose a BCR recovery method to recover and amplify
discovered hits of interest.
| Cell | Opto® B Discovery cDNA Synthesis Kit (750-02030) |
| OptoSeq® BCR | OptoSeq® BCR Kit v2 (750-08238) Optional Extended OptoSeq® BCR Bead Unload Kit (750-08097) |
| OptoSeq® Barcoded BCR | OptoSeq® Barcoded BCR Kit (750-01005) Optional Extended OptoSeq® BCR Bead Unload Kit (750-08097) |
5) Sequencing Library Prep Kit
Used for library preparation for downstream BCR sequencing.
| Mouse | Opto® B Discovery Sanger Prep Kit, Mouse (750-01004) OptoSeq® Barcoded BCR NGS Prep Kit, Mouse (750-02052) |
| Human | Opto® B Discovery Sanger Prep Kit, Human (750-02041) |
| Rabbit | Opto® B Discovery Sanger Prep Kit, Rabbit (750-08257) |
| Alpaca | Opto® B Discovery Sanger Prep Kit, Camelid (KT52-00010) |
Order Your Antibody
Discovery Kit Today
Explore Antibody Discovery
Applications
Beacon for Antibody Discovery
Unleash the Full Potential of
Your Antibody Discovery Research
Complete the form to connect with us and discover how the Beacon technology can enhance your Antibody Discovery programs.
Our expert team will be able to answer your questions, show you how the Beacon system works, and share the most relevant datasheets & case studies for your research.