Each cell has a phenome & is an independent functioning life.
The phenome is the sum of many functions, or collective life, for each cell.
Bruker Cellular Analysis provides missing insights into the true in vivo biology (i.e. the phenome) of each cell required to advance discovery to clinical research.
FUNCTIONAL CELL BIOLOGY
The Phenome, Defined
The phenome is the set of all phenotypic traits expressed by a cell or organism, central to all questions in biology, in which the sum of the parts creates a whole.
“Life’s definition, as it stands now, is akin to a menu. It is not one thing but a series of things, a set of behaviors, a series of processes, not a single property. To be living, an organism must have the capacity to reproduce, to grow, to metabolize, to adapt to stimuli, and to maintain its internal milieu.”
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Key Takeaways
The evolution of functional biology has brought us to this current era of the phenome.
Traditional technologies parse individual parts of the phenome but do not provide the whole picture.
Bruker Cellular Analysis empowers researchers to leverage the full potential of each cell and drive the next era of functional biology.
Overview
A New Phenome Defined
The evolution of functional biology began with immune surface breakthroughs and the advent of flow cytometry, which defined CD4 T cells and enhanced the ability to combat infectious diseases. Following this, significant genome breakthroughs led to the ability to change T cell receptors and create novel therapies such as CAR-T. The era of phenome is now upon us as we increase our scope of biological understanding, enabling the engineering of new therapies with optimal functional phenomes.
THE ERA OF THE PHENOME
An Evolution of Biological Breakthroughs:
The Era of the Phenome in Historical Context
The Challenge: A Disconnected View of Biology
A Disconnected View
In particular, today's solutions typically look at three things:
- Parts of each cell's phenome
- Across multiple instruments
- Creating slowness and complexity to leverage the phenome
Bruker Cellular Analysis is Designed to Enable the Next Wave of Biological Breakthroughs
Our technologies uniquely capture aspects of the phenome required to accelerate biological solutions for thousands of labs in this next era of the phenome.
The Bruker Cellular Analysis Suite of discovery to clinical research technologies can capture the phenome, the collective sum of the parts of the cells, the phenotype, and the functional phenotype, and leverage that to pick the best whole cells and pick the best patient response.
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Key Takeaways
Traditional technology misses the critical functional attributes that only Bruker Cellular Analysis' unique highly-functional biology can reveal.
Bruker Cellular Analysis' functional difference uniquely reveals the highly-proteomic and multi-functional immune cells orchestrating immune response.
Potent cells are key predictors of early and persistent response to cancer, vaccine efficacy, and overall survival, as published in various high-impact journals.
Overview
Our Highly-Functional Cells
Bruker Cellular Analysis uniquely reveals Potent and Dysfunctional Cells — highly polyfunctional cells that are early actors and orchestrators in the immune response. These rare subsets of highly functional cells can be beneficial (Potent Cells) or detrimental (Dysfunctional Cells). Detecting these cells is critical for developing more effective immune therapies and vaccines.
Our Most Potent Cells
Bruker Cellular Analysis: Our Most Functionally Powerful Cells, Revealed
Potent Cells
Potent cells are rare subsets of highly-polyfunctional cells that orchestrate the response against tumors and correlate to potency, persistence, and survival.
Dysfunctional Cells
Dysfunctional cells are highly-polyfunctional cells that drive disease progression and correlate to inflammation, toxicity, and resistance.
Why Proteomic Function is Critical to Potent Cells
The proteome is complex and requires direct detection. Dynamic in vivo biology is the captured by the proteome— the key to advancing medicines and diagnostics.
Unique Potent Cells are Key to Fighting Cancer
Bruker Cellular Analysis technology can uniquely reveal highly functional cells — the 5-10% of cells that are early actors of an immune response. We achieve this by detecting each cell's highly multiplexing cytokines, chemokines, and phosphoproteins.
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Key Takeaways
Polyfunctional cells secrete many different cytokines simultaneously and have correlated with outcome in multiple studies.
The Next Wave of Biology & Medicine is the Era of the Phenome — Bruker Cellular Analysis will power all labs across that new frontier.
Overview
Our Leap in Innovation
The IsoSpark's leap in innovation allows researchers to immediately detect the most potent immune cells and leverage that response data for preclinical and translational/clinical research.
The Beacon's breakthrough single-cell functional phenotyping tells you exactly which immune/production cell functionally produces key antibodies/proteins and the receptor/clone data, with the ability to retrieve that live cell.
This leap in innovation allows researchers to immediately detect the most potent cell and leverage it for discovery and bioprocessing.
OUR INNOVATIVE TECHNOLOGY
Why Understanding Single Cell Function is Critical
Cytokine function dictates the response of each cell to the tumor:
- CD4 T cells: Cytokines orchestrate the rest of the cells to attack the tumor
- CD8 T cells & NK Cells: Cytokines deliver the payload to the tumor
- Macrophages & T-Reg: Cytokines drive immune suppression in the tumor
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Directly Detect the Potent Functional Phenome of Each Cell
With the leaders from Kite Pharma and NCI: in a 20-patient non-Hodgkin Lymphoma CAR-T study, the frequency of highly functional cells (~20-25%), i.e., High PSI of each CAR-T cell therapy product, predicted response - Flow Cytometry & Bulk ELISA did not. Functional single-cell biology uniquely provides this information and can be targeted to create better products before release.
Directly detect the Best Antibodies and Most Potent Cells
Emergency Use Authorization of AstraZeneca’s Evuseld: Researchers at Vanderbilt used our Optofluidic platform to discover the antibodies from primary human tissues. Patients given a single injection of the antibody treatment were 83% less likely to develop symptomatic cases of the coronavirus than participants who were given a placebo.
In a separate trial, patients with mild-to-moderate COVID-19 who were given one dose of antibody treatment within three days of developing symptoms had a reduced risk of developing severe disease by 88%.
Results published in Nature Medicine.
Directly Discover the B cells Producing the Most Potent Antibodies
Every B cell can produce a unique antibody, and discovering the most potent antibody often requires finding the rate B cell in a highly diverse cell population.
Antibody discovery using the traditional hybridoma approach requires the immortalization of B cells by cell fusion that results in the death of >90% of B cells. This significantly reduces the chances of discovering novel antibodies against difficult targets.
Opto B Discovery workflows on the Beacon systems increase the probability of finding the best antibody by direct functional assessment of antibody products by single B cells.
BRUKER CELLULAR ANALYSIS INNOVATION IN CELLULAR ANALYSIS
The IsoCode Chip
Our proprietary & patented “Proteomic Barcoded” IsoCode Chip, named the Scientist's number #1 innovation, works by detecting 30+ cytokines per cell, measuring the functional phenotype of each immune cell.
This breakthrough technical innovation allows for complete single-cell functional characterization — something that was not possible before.
The OptoSelect Chip
Our OEP fluidics uses light and millions of light-actuated pixels to move individual cells to be isolated, cultured, assayed, and exported. Each cell or clone is imaged and monitored in real-time in a NanoPen™ chamber on our OptoSelect™ chips to provide rich visual data early and often. Our Optofluidic product suite delivers cell processing and deep profiling with more information about cell function than any other technology.
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The Functional Cell Biology Company
Join researchers worldwide using Bruker Cellular Analysis to reclaim lab time, save precious samples, increase efficacy, and cut down therapeutic timelines by a year+.
Novel Insights Across Applications
Gain deeper access to in vivo biology through highly-multiplexed functional cell biology.
Generate Publication-Ready Visualizations
Instantly analyze, dissect, visualize, and export your data with a click of a button — no coding required.
Save Time with Walk-Away Automation
Fully automate your workflow with our all-in-one, intuitive system that fits on your lab bench.
EXPAND
Cytokines Define Cell Function
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