Tech Note
Opto® B Discovery: Relative Affinity Ranking for Soluble Antigens
This document outlines the materials, methods, and experimental design recommended for performing Capture Assays on antibody-secreting B cells. These assays are designed to be compatible with calculating Beacon® Affinity Scores used for Relative Affinity Ranking for Soluble Antigens.
What's Inside
In this Tech Note we discuss:
Reagent and assay designs compatible with the Relative Affinity Ranking algorithm
Instructions for calculating the Beacon Affinity Score from antigen and IgG bloom scores
Materials and methods for fluorescently labeling antigens compatible with Relative Affinity Ranking
Guidance on multiplexing and assay design for Relative Affinity Ranking