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Training Modules
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18 Chapters
IsoSpeak Data Analysis and Visualization
Learn how IsoSpeak empowers users with the ability to explore their high dimensional functional data interactively.
Explore by Chapter
![](https://isoplexis.com/wp-content/uploads/2020/07/isou-icon-down-right-arrow@2x.png)
Explore by Chapter
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/Chapter1.png)
CHAPTER 1
0:50
Getting Started
Explore your high dimensional functional data with IsoSpeak.
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/chapter-2-2.png)
CHAPTER 2
0:57
Sample Validation using IsoQ Score
Analyze and validate cell sample quality using the IsoQ Score.
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/chapter-3-2.png)
CHAPTER 3
1:01
Analytics Interface Overview
Navigate the user interface and customization options in IsoSpeak.
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/chapter-4-2.png)
CHAPTER 4
1:07
Analytics Interface: Biomarker Analysis & Data Vizualization
Explore the data visualization options available in IsoSpeak.
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/chapter-5-1-1.png)
CHAPTER 5
1:22
Single Cell PAT-PCA
Generate and export a Single Cell PAT-PCA.
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/chapter-6-1-1.png)
CHAPTER 6
1:11
Single-Cell Polyfunctional Heatmap
Generate and export a Single Cell Polyfunctional Heat Map.
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/chapter-7-1-1.png)
CHAPTER 7
1:12
Single-Cell Polyfunctional Overview
Generate and export a Single Cell Polyfunctional Overview.
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/chapter-8-1-1.png)
CHAPTER 8
1:21
Single-Cell Polyfunctional Strength Index (PSI)
Generate and export a Single Cell Polyfunctional Strength Index (PSI).
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/chapter-9-1-1.png)
CHAPTER 9
1:17
Single-Cell Polyfunctional Strength Index Individual Cytokine Contribution
Generate and export a Single Cell Polyfunctional Strength Index (PSI) measuring individual cytokine contributions.
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/chapter-10-2.png)
CHAPTER 10
3:20
Mapping 3D t-SNE
Generate and export a single cell polyfunctional 3D t-SNE for single cell mapping.
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/chapter-11-umap.png)
CHAPTER 11
2:21
Generating a 3D UMAP
Generate and export a 3D UMAP in IsoSpeak
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/chapter-12A-signal-intensity-heatmap.png)
CHAPTER 12A
1:23
Single-Cell Intracellular Proteome Visualizations, Part A: Signal Intensity Heatmap
Generate and export a signal intensity heatmap in IsoSpeak
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/chapter-12B-violin-plot.png)
CHAPTER 12B
1:23
Single-Cell Intracellular Proteome Visualizations, Part B: Signal Intensity Violin Plot
Generate and export a signal violin plot in IsoSpeak
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/chapter-12C-2d-tsne.png)
CHAPTER 12C
1:18
Single-Cell Intracellular Proteome Visualizations, Part C: 2D t-SNE
Generate and export a 2D t-SNE in IsoSpeak
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/chapter-12D-chord-diagram.png)
CHAPTER 12D
1:04
Single-Cell Intracellular Proteome Visualizations, Part D: Correlation Chord Diagram
Generate and export a correlation chord diagram in IsoSpeak
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/chapter-13-exporting-data.png)
CHAPTER 13
0:56
Exporting Data
Exporting your IsoSpeak data
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/Screenshot_1.png)
CHAPTER 14 Appendix
5:06
Field Guide - Scientific Support
Initiating scientific support from PhenomeX
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/isospeak-appendix-thumb.jpg)
CHAPTER 15 Appendix
5:17
Field Guide - Analyzing Your Data
Analyzing your data with IsoSpeak