Enabling iPSC Cell Line Development Using Bruker’s Optofluidic Systems

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App Note

Bruker’s optofluidic technology enables manipulation and characterization of induced pluripotent stem cells (iPSCs) at the single-cell level, providing the opportunity to accelerate iPSC cell line development.

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What's Inside

In this App Note we discuss:

A streamlined workflow that enables single iPSC isolation, culture, and recovery using Bruker’s optofluidic systems.

Retention of positive pluripotent markers via immunocytochemistry following clonal export and off-chip expansion.

A customized and yet generalizable surface coating method for OptoSelect^® chips to support specific cell culture needs.

Successful clonal iPSC expansion on-chip.

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