This study demonstrates the robustness, reproducibility, and functional depth of the Opto® Cell Therapy Development Workflow for single-cell T-cell analysis using BiTE engagement on the Beacon® platform.
What's Inside
In this Datasheet we discuss:
Bulk assays mask exact effector identity and have confounding paracrine signals, while the Beacon system isolates single cells, enabling direct measurement of cytotoxicity, cytokine secretion, and activation from individual T cell–target–BiTE interactions
Key functional readouts exhibited consistent performance within expected variability ranges
Comparisons with bulk co-culture assays highlighted the advantages of single-cell resolution
