Beacon Select™ for
Cell Line Development
Our award-winning optofluidic technology designed specifically
for cell line development, now scaled to meet your needs and budget
High Quality, Value-Driven Cell Line Development
The Beacon Select for Cell Line Development optofluidic system offers a cutting-edge 2-nest solution to compress cell line development timelines, enabling faster cloning, screening, and selection of CHO cell lines.
- Boost efficiency by reducing development time by 50% and increasing throughput by 4x, while ensuring >99% monoclonality for regulatory filings.
- Run up to 2 chips at once
- Obtain high-throughput single-cell cloning and quality screening in just days with the ability to run up to 2 chips at once
- Quickly identify clones with desirable product quality attributes by running a variety of assays, including monoclonality, quality, and productivity, reducing the risk of late-stage failures and improving success rates during the scale-up process
- Streamline clone identification with essential assays like monoclonality, quality, and productivity, reducing the risk of late-stage challenges and optimizing scale-up success rates
- Accelerate your cell line development timelines while maintaining high quality and cost-effectiveness
The Leading Functional Single Cell
Platform for Cell Line Development
Function-First Total Solution for In-Depth Cell Line Development
At the core of the Beacon system is a combination of optics and nanofluidics called optofluidics. The OptoSelect® chips replace typical well plates. Each OptoSelect chip contains thousands of NanoPen® chambers, which replace wells on a microplate. This is where cells are selectively placed, cultured and characterized using a myriad of proprietary Beacon Platform assays.
Key Product Features
- Mid-level throughput with 2 nests that allows for up to 2 chips at a time
- Positive pressure to protect the sample from contamination
- Comprehensive assays to assess monoclonality, quality, and productivity
- Selective cell cloning to improve clone productivity
- Live cell recovery for export
- Rapid, automated single-day workflow
- Ability to assess multiple proteins, including monoclonal antibodies, bi-specific antibodies, and other therapeutic proteins
Unique Features of the Beacon Technology
At the core of the Beacon technology is the OptoSelect® chip, which combines nanofluidics and opto-electropositioning technology, referred to as optofluidics. Optofluidics is able to move single cells or beads in large numbers so they can be isolated, cultured, assayed, and exported. Single cells with phenotypes of interest can then be recovered for downstream analyses such as sequencing.
Provides Exquisite Control of Cells and Beads
- Uses light to move cells around and into the NanoPens
- Allows for thousands of parallel single cell experiments at once
Allows Continuous Media Perfusion
- Provides minimal disruption of the contents in the NanoPens
- Allows for exchange of oxygen, nutrients, and waste in and out of the NanoPens in a matter of seconds
- Keeps cells alive and healthy for days to weeks
High Throughput Chips
Depending on your assay and throughput needs, chips with different throughput and NanoPen chamber sizes are available for the Beacon Select for Cell Line Development system, and you can run up to 2 chips at the same time.
OptoSelect 1750 Chip
1.7 nL NanoPen chambers are ideal for longer term culture experiments including cell line development and adherent cell cloning.
Automatically and Precisely
4 Simple Steps to Your Best Cells
OEP fluidics uses light and millions of light-actuated pixels to move individual cells so they can be isolated, cultured, assayed and exported. Each cell or clone is imaged and monitored in real-time in a NanoPen® chamber on our OptoSelect® chips to provide rich visual data early and often. Our full platform and software suite deliver cell processing and deep profiling with more information about cell function than any other technology.
1. Sort
Our software automatically detects individual cells and then uses patterned light to isolate single cells into NanoPen chambers.
2. Culture
As cells expand fresh media is perfused across the chip, nutrients diffuse in and waste diffuses out while the software images the NanoPen chambers continuously – enabling cell counting, growth rate calculations, or other morphological characterization.
3. Assay
Run thousands of assays in parallel using our NanoPen chambers that are 100,000 times smaller in volume than a microwell in a 96 well plate – enabling phenotypic characterization of individual or populations of cells.
4. Recover
Selectively recover your best cells with phenotypes of interest from NanoPen chambers for downstream processing including sequencing or live cell recovery.
Simultaneous Incubation and Screening of Thousands of Clones
The Opto Cell Line Development workflow allows for selection of clonal cell lines with optimal growth, productivity and quality profiles, using bioreactor-relevant on-chip assays. During the Opto Cell Line Development workflow, transfected cells are enriched, cloned, cultured, assayed, selected and recovered in a single automated process with minimal human intervention. This is compared to conventional clone selection methods where multiple steps must be performed sequentially on many different instruments.
Selection Based on Product Quality, Not Just Productivity
The Opto Assure assays minimize the risk of costly late stage failures by identifying top producing clones with desirable product quality attributes early on. The Opto Assure assay for aggregation enables direct detection of product aggregates within days of single-cell cloning. This lets you confidently identify and select clones that secrete high-quality complex molecules and develop better production cell lines faster.
Customize Your Assays for Non-Antibody Proteins
Our Custom Productivity Assay enables selection of clones secreting a wide variety of non-antibody molecules. Compatible with the Beacon Select system, the Custom Productivity Assay uses labeled detection molecules to measure a wide range of secreted proteins, including antibodies, protein-based vaccines, enzymes, fusion proteins, antibody fragments and cytokines.
Dive Deeper Into the Data With Powerful Software
Cell Analysis Suite (CAS®) runs all assay operations, from importing samples to exporting live cells of interest.
Easy drag and drop workflow builder for flexible assay setup.
Cell Analysis Suite (CAS®)
Design and execute single cell experiments in real-time
with unparalleled flexibility to generate and
analyze functional data at scale.
Assay Analyzer (AA)
Filter, sort and visualize individual pens to review the history of each clone throughout your workflows and downselect cells or NanoPen chambers of interest. Compile your images and data into a PDF report with your new findings.
Image Analyzer (IA)
Score assays, count cells, consolidate images, view and process time lapse images, and perform pseudo coloring with this image visualization application.
with the Beacon Platform
Opto® CLD Workflow
The Opto CLD Workflow reduces the number of clones that are moved into scale-up and increases confidence in the clones that have been selected.
Beacon System
Traditional
Target to Lead Molecule in Weeks, Not Months
Selective Cell Cloning can be used to reduce CLD timelines by gently sorting a pool of cells 2.5 weeks post transfection
when cell viabilities are as low as 30%.
Researcher Spotlights
Unleash the Full Potential of
Your Cell Line Development
Complete the form to connect with us and discover how the Beacon technology can enhance your Cell Line Development Programs.
Our expert team will be able to answer your questions, show you how the Beacon system works, and share the most relevant datasheets & case studies for your research.