Protocol Training Modules
14 Chapters
IsoCode Single Cell Polyfunctional Strength: PBMC Protocol
Follow along with the validated PBMC Sample Preparation Protocol.
Explore by Chapter
Explore by Chapter
CHAPTER 1
1:29
Getting Started
Performing sample preparation for the IsoCode Single Cell Polyfunctional Strength PBMC Protocol
CHAPTER 2
1:14
Coat Culture Plates with Anti CD3
We’ll begin with coating our culture plate with Anti CD3
CHAPTER 3
2:56
Recovery of Cryopreserved Cells
Recovering your cryopreserved cells
CHAPTER 4
1:38
Pre-Sample Enrichment
Preparing your cells for sample enrichment
CHAPTER 5
6:06
CD8 Sample Enrichment
Enriching for CD8 T Cells
CHAPTER 6
5:34
CD4 Sample Enrichment
Enriching for CD4 T Cells
CHAPTER 7
1:00
Preparation of Stimulation Plate
Preparing your culture plate for cell stimulation
CHAPTER 8
2:04
Cell Stimulation
Instructions on how to begin stimulating your cells
CHAPTER 9
0:39
Chip Thaw
Thawing of the IsoCode Chips 30-60 minutes prior to cell loading
CHAPTER 10
4:18
Cell Staining
Staining your stimulated cells
CHAPTER 11
1:46
Chip Loading
Loading your stained and stimulated cells into the thawed IsoCode Chips
CHAPTER 14 Appendix
3:36
Field Guide - Removing Adherent Monocytes from a Culture Flask
Watch Field Guide: Removing Adherent Monocytes from a Culture Flask
CHAPTER 13 Appendix
3:37
Field Guide - How to Lift Stimulated CD8/CD4 Cells
Watch the Field Guide: How to Lift Stimulated CD8/CD4 Cells
CHAPTER 12 Appendix
3:53
Cell Quantification & Viability
Calculating cell quantification and viability.