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Protocol Training Modules
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14 Chapters
IsoCode Single Cell Polyfunctional Strength: PBMC Protocol
Follow along with the validated PBMC Sample Preparation Protocol.
Explore by Chapter
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Explore by Chapter
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/chapter-1.png)
CHAPTER 1
1:29
Getting Started
Performing sample preparation for the IsoCode Single Cell Polyfunctional Strength PBMC Protocol
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/chapter-2.png)
CHAPTER 2
1:14
Coat Culture Plates with Anti CD3
We’ll begin with coating our culture plate with Anti CD3
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/chapter-3.png)
CHAPTER 3
2:56
Recovery of Cryopreserved Cells
Recovering your cryopreserved cells
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/chapter-4.png)
CHAPTER 4
1:38
Pre-Sample Enrichment
Preparing your cells for sample enrichment
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/chapter-5.png)
CHAPTER 5
6:06
CD8 Sample Enrichment
Enriching for CD8 T Cells
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/chapter-6.png)
CHAPTER 6
5:34
CD4 Sample Enrichment
Enriching for CD4 T Cells
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/chapter-7.png)
CHAPTER 7
1:00
Preparation of Stimulation Plate
Preparing your culture plate for cell stimulation
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/chapter-8.png)
CHAPTER 8
2:04
Cell Stimulation
Instructions on how to begin stimulating your cells
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/chapter-9.png)
CHAPTER 9
0:39
Chip Thaw
Thawing of the IsoCode Chips 30-60 minutes prior to cell loading
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/chapter-10.png)
CHAPTER 10
4:18
Cell Staining
Staining your stimulated cells
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/chapter-11.png)
CHAPTER 11
1:46
Chip Loading
Loading your stained and stimulated cells into the thawed IsoCode Chips
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/monocyte-thumbnail.png)
CHAPTER 14 Appendix
3:36
Field Guide - Removing Adherent Monocytes from a Culture Flask
Watch Field Guide: Removing Adherent Monocytes from a Culture Flask
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/vid-thumb-field-guide-how-to-lift-stimulated-cd8-cd4-cells.jpg)
CHAPTER 13 Appendix
3:37
Field Guide - How to Lift Stimulated CD8/CD4 Cells
Watch the Field Guide: How to Lift Stimulated CD8/CD4 Cells
![](https://brukercellularanalysis.com/wp-content/uploads/2023/03/cell-count.png)
CHAPTER 12 Appendix
3:53
Cell Quantification & Viability
Calculating cell quantification and viability.