Biological and technical variation in IsoLight single-cell proteomics experiments

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Tech Note

Explore technical and biological sources of variation and effects on single-cell cytokine secretion data on the IsoLight system.

What's Inside

In this Tech Note we discuss:

Replicate consistency: correlation of t-SNE cell subsets

Sample-to-sample correlation: consistency of secretion intensity across all 32 measured cytokines

Sample-to-sample correlations: split view

A Deeper Look

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Single-Cell Proteomics Experiments

Technical and biological sources of variation were evaluated for their effects on single-cell cytokine secretion data from the IsoLight platform.

The nearly identical resulting single-cell t-SNE plots and single-cell intensity levels across all four replicates indicate very low levels of variability.

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